Recalibration
HTL pipettes are calibrated by gravimetric method, using
distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard. If during pipette
operation you find that the accuracy error (the difference
between the real aspirated volume and the preset volume)
exceeds the permissible value given in the
Instruction
Manual, the pipette recalibration procedure should be carried out.
Before starting the recalibration it is necessary to
check whether the following requirements have been fulfilled
during error determination:
- the ambient temperature, and the temperature of the
pipette, tips and water was identical
- the density of the liquid used is close to that of
distilled water
- a balance with appropriate sensitivity has been used
Volume
tested
(µl) |
Measurement
Accuracy
(mg) |
Standard
Deviation
(mg) |
| ≥
0,1-10 |
≤ 0,001 |
≤ 0,002 |
| ≥ 10-100 |
≤ 0,01 |
≤ 0,02 |
| ≥
100-1000 |
≤ 0,1 |
≤ 0,2 |
| ≥ 1000 |
≤ 0,1 |
≤ 0,2 |
- mg/µl conversion factor has been taken into
account
- the requirements given in sections Aspirating and Recommendations of Instruction
Manual have been fulfilled.
If the above conditions are satisfied and the accuracy
error for selected volume exceeds the permissible value -
see in
Instruction
Manual,
the pipette recalibration procedure should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
Recalibration conditions
- Ambient temperature and the temperature of the pipette,
tips and liquid should be within the range 20-25°C
and stabilized during weighing within ±0.5°C
- Measurements should be conducted using distilled
water
- Balance sensitivity should be suitable for the volume
to
be controlled.
Step by step recalibration procedure
Click on the appropriate Pipette Serie:
|
